International Journal of Environmental Science and Development

Abstract—The isolation of 17α-Ethinylestradiol (EE2)-degrading bacterial strains was done using samples sourced from Palm Oil Mill Effluent (POME) and fresh cow dung. Screening processes were conducted to identify the potential strains. The three highest potential EE2-degrading bacterial strains obtained from each sample source were selected for further batch experiments. A batch study with the strains fed with only salt medium containing EE2 as the sole carbon source was conducted for 9 days. The bacterial strain isolated from POME that was capable of degrading EE2 of up to 64.0 % at EE2 concentration of 5 mg/L was identified as Hyphomicrobium facile. The strain isolated from the cow faeces that was capable of degrading EE2 up to 55.1 % at concentration of 10 mg/L was identified as Enterobacter cloacae.

Index Terms—17α- ethinylestradiol, cow dung, Enterobacter cloacae, Hyphomicrobium facile, palm oil mill effluent.

The authors are with University Sains Malaysia, Environmental Technology Division, Penang, 11800 Malaysia (e-mail: yeeling_77@yahoo.com, nhashima@usm.my, japareng@usm.my, cynthia_sze91@hotmail.com, echa2330@yahoo.com).

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Cite: Yee Ling Lim, Norhashimah Morad, Japareng bin Lalung, Sze Yie Chan, and Siti Aishah Abu Bakar, "Isolation and Identification of 17α- Ethinylestradiol — Degrading Bacterial Strains from POME and Cow Dung," International Journal of Environmental Science and Development vol. 7, no. 12, pp. 881-884, 2016.