International Journal of Environmental Science and Development

Abstract—Thai degrading-bacteria, strain ARB2, was isolated from sugarcane field soils in Ratchaburi province of Thailand. Strain ARB2 was gram-negative bacteria, rod shape in pair and a single cell. The genomic DNA was extracted following the standard protocol for bacterial genomic DNA preparations. The partial 16S rDNA genes were amplified by polymerase chain reaction (PCR) using the universal primers of 16S rDNA gene. Sequence analysis of the PCR product indicated that the 16S rRNA gene in strain ARB2 was ranging from 89-91% identical to the same region in Xanthomonas sp. and were named Xanthomonas sp. ARB2. Xanthomonas sp. ARB2 was capable of degrading 300 mg L^-1 and 100 mg kg^-1 of atrazine in mineral salts liquid medium and soil at 81% and 62% in 7 days, respectively. GC-MS analysis detected the formation of two metabolites, deethylatrazine (DEA) and deisopropylatrazine (DIA) during the process of degradation of atrazine.

Index Terms—Atrazine, biodegradation, bioremediation, contaminated soil.

Sopid Sawangjit is with the Department of Biotechnology, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok, CO 13000, Thailand (e-mail: sopid.sa@ssru.ac.th, sawangjit.s@hotmail.com).

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Cite: Sopid Sawangjit, "Isolation and Characterization of Atrazine-Degrading Xanthomonas sp. ARB2 and Its Use in Bioremediation of Contaminated Soils," International Journal of Environmental Science and Development vol. 7, no. 5, pp. 351-354, 2016.