International Journal of Environmental Science and Development

Abstract—In the suspension culture, the sharp structure of some nano-metals may destroy the cells and cause metal washout from the system, causing secondary pollution. A novel method of combination of nano-metals addition and immobilized dark fermentation microorganism can overcome this problem. In this study, the co-immobilization approach, nano-metal and the dark-fermented bacteria were incubated together within PVA-boric acid gel granule, could prevent washout of hydrogen producing microorganism as well as the added nano-metals. The results showed when 400 mg/L nano-metal nickel (NP-Ni) and 400 mg/L nano-metal iron (NP-Fe) were added within the immobilized cell, both the maximum H2 production yield (HPY) and H₂ production rate (HPR) were improved to be 1.28 mol H₂/mol glucose, 3.13 H₂ L/L/day and 1.19 mol H₂/mol glucose, 1.90 H₂ L/L/day, respectively. The method is expected not only to allow microorganisms to have higher environmental tolerance but also increase hydrogen production.

Index Terms—Hydrogen, dark fermentation, immobilization, nano-metal.

The authors are with the Department of Environmental Engineering, National Chung-Hsing University, Taiwan (e-mail: Anongnartwannapokin@gmail.com, wibbly520@gmail.com, james562443@gmail.com, stringbird@gmail.com, badger@nchu.edu.tw).

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Cite: Anongnart Wannapokin, Yu-Tzu Cheng, Sheng-Zhe Wu, Ping-Heng Hsieh, and Chun-Hsiung Hung, "Co-immobilization of Nano-Metal and C. pasteurianum for Dark Fermentation Anaerobic Hydrogen Production," International Journal of Environmental Science and Development vol. 10, no. 11, pp. 404-409, 2019.

Copyright © 2019 by the authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).