International Journal of Environmental Science and Development

Abstract—To enhance lipopetide biosurfactant production by Pseudomonas spp., the effects of medium and culture conditions were investigated. Nutrient broth (NB) supplemented with glucose and molasses as carbon sources were used to increase biosurfactant yield. Cultivation temperatures and initial pH were also studied. In this experiment, NB was used as a control to produce biosurfactant with the yield of 0.58 g/L. The lipopeptide biosurfactant yield was increased to 2.76 g/L when Pseudomonas spp. was cultured in NB supplemented with 1% w/v glucose, 500 μM FeSO4, pH 9.0 and cultivation of 20°C for 72 h. Its specific growth rate was 0.25 h-1.

Index Terms—Pseudomonas spp., lipopeptide biosurfactant, glucose, ferrous.

Thanawat Choopraserdchok and Pumnat Cheunchomrat are with the Department of Food Science and Technology, Faculty of Science and Technology, Thammasat University, Rangsit Campus, Pathum Thani, 12121 Thailand (e-mail: cpumnat@tu.ac.th).
Dusit Athinuwat is with the Department of Agricultural Technology, Faculty of Science and Technology, Thammasat University, Rangsit Campus, Pathum Thani, 12121 Thailand.

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Cite: Thanawat Choopraserdchok, Dusit Athinuwat, and Pumnat Cheunchomrat, "Effects of Glucose and Ferrous Supplements and Culture Conditions on Lipopeptide Biosurfactant from Pseudomonas spp.," International Journal of Environmental Science and Development vol. 8, no. 11, pp. 772-775, 2017.