International Journal of Environmental Science and Development

Abstract—In vitro somatic embryogenesis and subsequent plant regeneration was achieved in callus cultures derived from internode, node, shoot tip, petiole and leaf explant of teasle gourd plant on semi-solid Murashige and Skoog (MS) basal salts and growth regulators supplemented with 1.0mgl-1 BAP, 0.1mgl-1 NAA and 30 g l-1 (w/v) sucrose. Somatic embryos proliferated rapidly by somatic embryogenesis after 4 weeks. The embyogenic callus germinated on MS salts and growth regulators supplemented with 1.0mgl-1 BAP and 0.1mgl-1 NAA. The embryo-derived plantlets were transfered on half MS media with 0.3 mgl-1 IBA and sufficient rooting was achieved. Plantlets were acclimatized in the controlled environment.

Index Terms—Callus culture, Explant, Somatic embryogenesis & Teasle Gourd.

Professor, 1 Ph.D Student, Dept. of Crop Botany, Bangladesh Agricultural University, Mymensingh-2202, BANGLADESH

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Cite: Mohammad Abdul Karim and Shorif Uddin Ahmed, "Somatic Embryogenesis and Micropropagationin Teasle Gourd," International Journal of Environmental Science and Development vol. 1, no. 1, pp. 10-14, 2010.